Square centrifuges are utilized depending on the size, density, medium viscosity, and rotor speed. Gravitational forces within a solution cause particles with densities greater than the solvent to sink and those with densities less than the solvent to float to the top. Centrifugation separates particles inside a solution by taking advantage of even tiny changes in density.

Centrifugal force is created as the rotor rotates around a central axis, pushing particles away from the axis of rotation. Particles will sediment if the centrifugal force exceeds the buoyant forces of liquid media and the frictional forces produced by the particle.

Details

• Model Number: L500.
• CE, ISO, RoHS, and FSC certifications
• Steel as a material
• Maximum Speed: 5000 RPM
• Type: L600-a
• Maximum Speeds: 5000 RPM
• RCF Max: 4030*G
• Maximum Volume: 4*4*15ml
• Plus or minus for speed accuracy Range of the 30r/Min time setting: 1-99min
• Noise level: 65 dB (a)
• AC220V+-22V, 50/60Hz, 2A Power Supply
• Size: 380 x 500 x 300 mm Package Dimension: 465 x 660 x 350 mm Net Weight: 19 kg
• Carton for transportation
• ISO9001: 2008, ISO13485, CE, and Cence are the specifications. The HS Code is 8421199090.
• 20 000 PCS per year is the production capacity.

Product uses

The product can separate fluids, gases, or liquids based on density in various laboratories. Centrifuges are employed in clinical and research facilities to purify cells, organelles, viruses, proteins, and nucleic acids. The separation of different components of whole blood using a centrifuge is an example of its use in a clinical environment.

Serum or plasma is required for several assays and can be obtained through centrifugation. A full blood sample can clot at room temperature to get the serum. Centrifugation removes the clot from the sample, leaving a serum supernatant behind.

In contrast to serum, plasma is made from whole blood that has not been allowed to clot; it contains serum and clotting factors. A full blood sample is taken in tubes that have been anticoagulant-treated to get plasma. Cells are taken out of the plasma after centrifugation, leaving the plasma supernatant.

Balancing the Centrifuge

• Ensure the sample tubes are all filled equally. If additional tubes are needed, fill them with water or a liquid that has a density similar to the sample and make sure the mass is balanced to within 0.1 grams if possible.
• Add a tube of equal weight in the opposite position to each tube introduced into the rotor. This will guarantee that the rotor's center of gravity stays there.
• Turn the rotor 90 degrees, then add two more tubes opposite one another.

Important questions to consider before purchasing this item

• How many sample volumes are you using? A floor-standing variant with a bigger capacity and various rotor configurations can be the best choice for procedures involving large or fluctuating volumes.
• Are samples responsive to temperature? Square centrifuges with options for temperature control and cooling are needed.
• How much lab space is available concerning the footprint of the centrifuge?
• What is the greatest g-force that the centrifuge can produce? While ultracentrifuges are required to separate DNA and RNA, low-speed centrifuges are best for separating entire cells.
• Will clinical or blood bank samples be processed in the centrifuge? For these particular uses, there are cell washers or clinical models available.
• How much lab space is available for the footprint of the centrifuge?

Advantages

• 25 user-defined programs saved
• A variety of brackets fit tubes of various capacities
• Touch panel and LCD
• Automatically calculating RCF value
• Microprocessor control and a DC brushless motor; a special dampening structure to reduce vibration
• Soft touch lid closure on the centrifuge since the centrifuge cannot function with the door open

Conclusion

The size and brittleness of the particles in the sample determine the best centrifugation speed. The centrifugation speed increases with decreasing particle size. Bacteria are pelleted at faster speeds (2000–10,000 x g) and for more delicate samples, slower centrifugation speeds may be used. RCF is another crucial element that affects the effectiveness of separation.

RCF is proportional to the square of RPM and the rotor's radius. Centrifugation speeds of 1000 RPM for 5 minutes and 500 RPM for 10 minutes cannot be substituted because of the square dependence of RCF on RPM. The former generates a significantly greater RCF than the latter. Therefore, it's crucial to consider RCF when choosing the speed and duration of your centrifugation.